Figure 4. NsPEFs ablate HCC tumor in vivo.

The mice were randomly divided into four groups. Control group (without nsPEFs treatment) (CT, n = 7), single treatment with 300 pulses group (S300, n = 7), three treatments with 100 pulses group (T100,n = 8); surgical resection group (SR,n = 6). The first nsPEFs treatment was deliver ed on the same day when the tumor volumes were around 0.4 cm³. The tumor volume on the nsPEFs treatment day, one week post treatment and the two weeks post treatment were calculated (Figure 4). Before the treatment (day 0), there was no significant difference in the tumor volumes among four groups (around 0.4 cm³.). On day 8 post nsPEFs treatment, tumor volume in T100 (0.42±0.11) cm³ was significantly decreased compared to CT (1.25±0.63) cm³ and S300(0.87±0.71) cm³. On day 16, tumor volume in T100 (0.40±0.14) cm³ was significantly smaller than that in CT (1.73±0.51) cm³ and S300(0.91±0.48) cm³.