Figure 1. Effect of in in vivo inhibition of MEK and PI3K in combination on ERK and S6 phosphorylations.

(A) CCSP/− and CCSP/TGFa mice on Dox for 2 weeks were co-treated with PX-866 (3 mg/kg, every other day) and ARRY (37.5 mg/kg) either once a day (QD) or twice a day (BID) for 7 days. Total lung lysates that represent four separate mice in each group were analyzed by immunobloting. Western blot analysis was used to determine levels of phosphorylated ERK (B) and phosphorylated S6 (C) that normalized to the total ERK and S6 protein in lung homogenates, respectively. Data are means ± SEM, and statistical significance between groups was measured using one-way ANNOVA (n = 4/group).