Table 2.
Efficiencies of miRNAs targeting the FMDV IRES in inhibiting EGFP expression in BHK-21 cells as assayed by flow cytometry
| Reporter plasmid |
Inhibition efficiency of each miRNA (%) |
|||||
|---|---|---|---|---|---|---|
| pmiR153 | pmiR220 | pmiR242 | pmiR276 | Bi-miRNA | Dual-miRNA | |
| pHN/IRES-EGFP |
72.2% |
56.7% |
44.3% |
81.4% |
84.7% |
95.0% |
| pFC/IRES-EGFP |
38.7% |
47.9% |
71.4% |
60.5% |
88.3% |
96.6% |
| pJS/IRES-EGFP | 64.6% | 37.5% | 68.8% | 62.5% | 78.4% | 83.6% |
Bi-miRNA, a mixture of pmiR242 and pmiR276 plasmids; Dual-miRNA, a co-cistronic expression plasmid (pmiR242 + 276) containing two pre-miRNA (pmiR242 and pmiR276) hairpin structures. Percentage inhibition in each co-transfected (vector-delivered miRNAs and the reporter plasmids) cell population was calculated by comparison with the control cells transfected only with the same reporter plasmid at 48 h post-transfection.