Figure 7.

LNA end-blocked ODN decoys containing internal α-l-LNA substitutions: design and estimation of the IC₅₀ values. C*+C* and C2*+C2* ODN decoys, in which the internal LNA monomers present in the C+C and C2+C2 molecules, respectively, were replaced with α-l-LNA nucleotides (bold upper case letters with asterisk in light grey) were synthesized and tested in gel shift competition experiments. Only the protein/probe complex is shown. Competitor concentrations in lanes 2–14 were 38, 57, 85.8, 128.7, 193, 289.6, 434.4, 651.5, 977, 1466, 2200, 3300 and 4950 nM. In lanes 1, 100% binding of NF-κB to the radiolabeled probe is shown. Lower case, DNA monomers; bold upper case in grey, LNA bases. IC₅₀ values of these molecules were determined by assaying a broader range of concentrations with respect to those shown in the figure. Data obtained were plotted as a function of decoy ODN concentration (log₁₀ [nM]) and fitted to a dose– response function (graph on the bottom). The 50% inhibitory concentrations (nM) ± SE are reported in Table 1. In the graph, the unmodified D+D ODN shown in Figure 4 is reported again to allow direct comparison.