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. 2014 Jan 27;9(1):e86882. doi: 10.1371/journal.pone.0086882

Table 2. Gene-specific primers used in real-time qRT-PCR analysis.

AGI code Target gene Primer sequences Product size (bp)
Salicylic acid regulated gene
At2g14610 PR-1 F5-TTCTTCCCTCGAAAGCTCAA-3 174
R 5-AAGGCCCACCAGAGTGTATG-3
At3g57260 PR-2 F 5 -AGCTTAGCCTCACCACCAATGT-3 83
R 5-CCGATTTGTCCAGCTGTGTG-3
At1g75040 PR-5 F 5-TGTTCATCACAAGCGGCATT-3 99
R5GTCCTTGACCGGCGAGAGTTAATGCCGC-3
Jasmonic acid/Ethylene regulated gene
At3g12500 PR-3 F 5-GGCCAGACTTCCCATGAAAC-3 113
R 5-CTTGAAACAGTAGCCCCATGAA-3
At3g04720 PR-4 F 5-GCAAGTGTTTAAGGGTGAAGAACA-3 104
R 5-GAACATTGCTACATCCAAATCCAAG-3
At5g44420 PDF1.2 F 5-TTTGCTGCTTTCGACGCAC-3 80
F 5-CGCAAACCCCTGACCATG-3
At5g24770 AtVSP2 F 5-TCAGTGACCGTTGGAAGTTGTG-3 104
R 5-GTTCGAACCATTAGGCTTCAATATG-3
At1g32460 MYC2 F 5-AGCAACGTTTACAAGCTTTGATTG-3 76
R 5-TCATACGACGGTTGCCAGAA-3
Housekeeping gene/internal control
At3g62250 UBQ5 F 5-GACGCTTCATCTCGTCC-3 256
R 5-GTAAACGTAGGTGAGTCCA-3
At2g37620 ACT2 F 5-AGTGGTCGTACAACCGGTATTGT-3 92
R 5-GATGGCATGAGGAAGAGAGAAAC-3