Skip to main content
. 2004 Mar 26;32(6):1917–1927. doi: 10.1093/nar/gkh511

Figure 2.

Figure 2

Vertebrate G-rich overhangs of at least 24 bases on telomeres of cells carrying Tlc1h. Non-denaturing in-gel hybridization was performed with human telomeric probes of various lengths. Shown here are the results obtained with [32P](C3TA2)4 (left) and [32P](C3TA2)5 (right) as probes. Gels were first washed at 30°C and, after appropriate exposures were obtained, they were rewashed at increasing temperatures as indicated on the sides of the panels. Lane 1: DNA derived from a strain carrying wt TLC1 and grown for 100 generations. Lane 2: DNA derived from a strain carrying Tlc1h and grown for 100 generations. Lane 3: linearized and denatured p16R (positive control with 1.6 kb of vertebrate repeats). Lane 4: linearized and denatured pTLC1hTRP (positive control with 19 nt of vertebrate repeats). Left: molecular weight standard. Solid arrows indicate the chromosomal terminal restriction fragments (TRFs); open arrows indicate the pTLC1hTRP control.