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. 2004 Mar 26;32(6):1917–1927. doi: 10.1093/nar/gkh511

Figure 5.

Figure 5

hTERT is localized to the yeast nucleus. In all panels, the nucleolar protein Nop1p was detected with a mouse monoclonal antibody against Nop1p and Oregon green-labelled goat anti-mouse antibody; hTERT fusion proteins were detected with a rabbit anti-hTERT polyclonal antibody and Texas red conjugated anti-rabbit antibody (red), and the nucleoplasm is represented in blue by DAPI staining.(A) control strain with no hTERT plasmid (BY4705 est2Δ/tlc1Δ cells with pEST2-LYS2, pTLC1hTRP and pRS422-hTR plasmids); (B) same as (A) plus pEGKT-hTERT plasmid; (C) same as (A) plus p426/CDC13DBD-hTERT plasmid. Representative fields with four cells are shown and identical results were also observed using strains that harbored TLC1wt instead of Tlc1h (data not shown).