Figure 1. Normalisations of Western blot replicates in the literature.

We divide the normalisations found in literature into three categories: (A) normalisation by fixed normalisation point or control; (B) normalisation by sum of the replicate; (C) normalisation by optimal alignment. For illustration purposes we do not use actual Western blot data. Each normalisation is presented using three cartoon Western blots, representing three replicates, and highlighting with red circles the data points used in the normalisation procedure. The graphs show the normalised data, where the points belonging to the same replicate are connected with lines.