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. 2014 Jan 27;9(1):e87228. doi: 10.1371/journal.pone.0087228

Figure 2. Inhibition of apoptosis in irradiated NSCs by BCL2-overexpression allows a more efficient astroglial differentiation.

Figure 2

(A)Vector-transduced or BCL2-overexpressing NSC were irradiated, apoptotic cell death analyzed by TUNEL assay. Error bars: SD. (B)Gene expression analysis at day 3 post irradiation of vector-transduced or BCL2-overexpressing NSC for the expression of stem cell marker Nestin, astrocyte markers GFAP and S100β, pro-apoptotic genes BAX and PUMA, normalized against respective non-irr cells. Technical triplicates, error bars: SD. (C)Western blot analysis of vector-transduced or BCL2-overexpressing NSC for expression of Nestin and GFAP following irradiation. Loading control: vinculin. (D)Gene expression analysis as in (A) for the expression of markers of mature neurons (TUJ1/β-tubulinIII) and oligodendrocytes (MBP), normalized against respective non-irr cells. Cave: both gene products were detected at rather low expression levels (high qPCR-cycle number). Technical triplicates, error bars: SD.