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. 2014 Jan 27;9(1):e87228. doi: 10.1371/journal.pone.0087228

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© 2014 Leonid Schneider

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A)Flow cytometry analysis for apoptosis-associated DNA fragmentation (Sub-G1) of irradiated NSCs, cultured in standard self-renewal medium, or supplemented with BMP2 (20 ng/ml), or switched to BDNF-containing Neuronal differentiation medium. Error bars: SD. (B)Representative immunofluorescence analysis of GFAP expression in non-irr and irr NSC subjected for 24 h to FCS. DNA was stained with DAPI. Magnification: 40×. (C)Gene expression analysis of astrocytes derived by 10% FCS from NSC (FCS d3), and when switched back to NSC culture medium, normalized against self-renewing NSC. Technical triplicates, error bars: SD. (D)TUNEL assay for irradiated NSC at day 3, cultured in unmodified (self-renewal promoting) medium or supplemented with FCS (astroglial differentiation medium), either immediately (d0+3) or 24 h after irr (d1+2). Error bars: SD.