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. 2014 Jan 27;9(1):e87323. doi: 10.1371/journal.pone.0087323

Figure 3. Effect of reevesioside A on the expression of several cell cycle regulators.

Figure 3

(A) PC-3 cells were incubated in the absence or presence of reevesioside A (50 nM) for various times. Cells were harvested and lysed for the detection of the indicated protein expression by Western blot analysis. The expression was quantified using the computerized image analysis system ImageQuant (Amersham Biosciences). The data are expressed as mean±SEM of three to five independent experiments. * P<0.05, ** P<0.01 and *** P<0.001 compared with 100% control. (B) After the treatment, the cells were harvested for immunoprecipitation assay. The protein expression was detected by Western blot analysis. Data are representative of three independent experiments.