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. 2014 Jan 27;9(1):e87508. doi: 10.1371/journal.pone.0087508

Figure 9. Real-time validation PCR for genes in LL and TEM GC gene lists.

Figure 9

ΔCTs plotted for 6 genes chosen for validation using the LL method of RNA extraction from blood (A). Four out of 6 genes (CDK1, EGR1, SSPN and IL23) could be validated from the Affymetrix microarray studies (C). ΔCTs plotted for 6 genes chosen for validation using the TEMPUS/GC method of RNA extraction from blood (B). Four out of 6 genes (EP400, E2F2, EHBP1 and KAZ) could be validated from the Affymetrix microarray studies (D). Blue bars represent levels of gene expression in ALS patients (n = 6) and red bars the levels in normal control individuals (n = 6).