Table 2. Selected QC metrics for PAX and TEM arrays.
SAMPLE | SF | RawQ | BG Avg | %P | Signal(All) | Actin Signal | Actin 3-5-ratio |
P13.mas5 | 23.86 | 0.832 | 29.48 | 33.74 | 910.39 | 41134.71 | 2.37 |
P16.mas5 | 20.97 | 0.805 | 30.11 | 33.61 | 908.23 | 35056.00 | 2.30 |
PG10.mas5 | 16.53 | 0.779 | 29.05 | 37.72 | 917.59 | 46817.15 | 2.25 |
PG11.mas5 | 26.34 | 0.784 | 29.39 | 32.91 | 975.39 | 58756.44 | 3.95 |
PG12.mas5 | 28.01 | 0.800 | 30.19 | 30.45 | 949.32 | 38274.88 | 3.03 |
PG14.mas5 | 20.69 | 0.773 | 29.40 | 34.20 | 937.87 | 49253.10 | 2.31 |
PG15.mas5 | 15.86 | 0.877 | 31.80 | 36.87 | 873.14 | 38324.91 | 2.23 |
PG17.mas5 | 13.24 | 0.792 | 29.55 | 39.35 | 848.18 | 33711.05 | 2.80 |
PG3_1.mas5 | 41.31 | 0.706 | 27.23 | 29.97 | 997.94 | 49350.14 | 31.60 |
PG4_1.mas5 | 51.73 | 0.744 | 28.46 | 26.93 | 1016.88 | 31745.37 | 48.67 |
PG5.mas5 | 17.60 | 0.769 | 28.84 | 36.57 | 959.76 | 45626.00 | 2.13 |
PG6.mas5 | 21.69 | 0.840 | 30.15 | 31.64 | 927.78 | 42699.89 | 3.18 |
PG7.mas5 | 12.57 | 0.930 | 32.50 | 38.57 | 867.41 | 33979.42 | 2.09 |
PG8.mas5 | 17.66 | 0.851 | 30.08 | 35.65 | 906.00 | 35655.77 | 2.38 |
PG9.mas5 | 16.20 | 0.839 | 31.17 | 37.20 | 886.68 | 24967.63 | 1.73 |
AV. PAX | 22.95 | 0.808 | 29.83 | 34.36 | 925.51 | 40356.83 | 7.53 |
T10.mas5 | 6.83 | 0.896 | 32.21 | 43.27 | 841.55 | 25843.87 | 1.67 |
T11.mas5 | 9.42 | 0.883 | 32.82 | 41.37 | 882.21 | 29647.80 | 1.42 |
T12.mas5 | 14.88 | 1.009 | 33.58 | 35.12 | 942.61 | 33275.39 | 1.37 |
T13.mas5 | 8.67 | 1.037 | 35.47 | 40.05 | 893.06 | 29685.47 | 1.38 |
T15.mas5 | 13.33 | 0.873 | 30.23 | 37.99 | 897.58 | 26182.47 | 1.60 |
T16.mas5 | 10.01 | 0.987 | 32.56 | 39.78 | 933.23 | 28284.56 | 1.25 |
T17.mas5 | 9.47 | 0.864 | 31.85 | 41.78 | 877.22 | 28296.21 | 1.23 |
T3_1.mas5 | 9.61 | 0.990 | 32.90 | 42.47 | 893.88 | 32139.52 | 1.46 |
T4.mas5 | 10.17 | 0.959 | 33.49 | 41.51 | 886.43 | 28244.28 | 1.44 |
T5.mas5 | 11.12 | 0.953 | 32.65 | 40.48 | 872.70 | 24144.52 | 1.66 |
T6.mas5 | 10.41 | 1.127 | 37.35 | 37.16 | 884.89 | 33334.57 | 1.30 |
T7.mas5 | 8.29 | 1.138 | 38.70 | 40.82 | 909.65 | 38670.56 | 1.40 |
T8.mas5 | 8.37 | 1.144 | 37.47 | 37.94 | 914.99 | 40451.85 | 1.26 |
T9.mas5 | 12.92 | 0.887 | 31.10 | 38.73 | 893.45 | 28633.24 | 1.56 |
AV. TEM | 10.25 | 0.982 | 33.74 | 39.89 | 894.53 | 30488.16 | 1.43 |
Scale factor (SF), rawQ, background (BG), percentage present (%P), total signal, actin signal, as well as actin 3′ to 5′ ratio are all listed for PAX and TEM arrays used in this study. Three arrays in total did not pass quality control and were not analysed: one array was extracted by the LL method, one array by TEM GC and one from TEM.