Table 4. PCR primer sequences designed for genes validated in this study.

LEUKOLOCK
Gene	Sequence	Product Size
FOXP1 S	ctctgtcatcacaaccaccag	138
AS	ggtggtctaacttctgcgttc
IL23R S	ggcaagaagtacttggtttgg	128
AS	atagtctcagccctggaaatg
CDK1 S	aggtcaagtggtagccatgaa	108
AS	tttggatgacgaagttccttt
EGR1 S	gagaaggtgctggtggagac	142
AS	cactgaccaagctgaagagg
MBNL3 S	tccatcaattccagctaatcc	112
AS	tccaggaatcagaacaggtg
SSPN S	ttacctgtgagaccacactcg	108
AS	cagtgacgctggtacagtcc

Samples of cDNA were diluted 1∶10, and 2.5 ul were used per PCR reaction for all genes except EHBP1, where 4 µl was used with 5x Brilliant III Ultra Fast SYBR Green QPCR Master Mix (Agilent). In all cases primer concentrations were optimised to 300 mM (S, sense strand; AS, antisense strand)