Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 27;9(1):e87517. doi: 10.1371/journal.pone.0087517

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Nejepinska et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Overlay of polysome profiles from HEK-293 cells transfected with either pCAGEGFP or pCAGEGFP-MosIR. (B) Polysome profile (upper part) and western blotting analysis (lower part) of respective fractions. The alignment of fractions on polysome profile and lanes on western blotting is highlighted by solid red lines flanking fractions 11 and 12. HEK-293 cells transfected with either pCAGEGFP or pCAGEGFP-MosIR were subjected to polysome profiling. Proteins were isolated from each fraction by ethanol precipitation and the same volume aliquots from each fraction were analyzed by polyacrylamide gel electrophoresis and western blotting. The last lane in each sample represents 1% of input. γ, phosphorylated form of protein; RPS14, ribosomal protein S14. The experiment was repeated three times, a representative result is shown.