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. 2014 Jan 27;9(1):e87519. doi: 10.1371/journal.pone.0087519

Figure 2. Detection of PLP-specific T cells by in situ staining with PLP 139–151 dextramers.

Figure 2

EAE was induced in SJL mice by immunizing the animals with PLP 139–151 in CFA. At termination, cerebrums collected from EAE mice were embedded in 4% agarose, and the sections were made using vibratome. After staining with cocktails containing either PLP 139–151 dextramers/anti-CD4 or TMEV 70–86 dextramers (control)/anti-CD4 and fixing with 4% PBS-buffered paraformaldehyde, sections were washed and mounted for examination by LSCM. Top panels: sections stained with PLP 139–151 dextramers and anti-CD4. Bottom panels: sections stained with TMEV 70–86 dextramers and anti-CD4. Left panel: CD4, green; Middle panel: dextramers, red; Right panel: merged (circles, dext+ CD4+ T cells; insets represent enlarged views of dext+ CD4+ T cells). Original magnification 1000×; bar  = 20 µm.