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. 2014 Jan 27;9(1):e87513. doi: 10.1371/journal.pone.0087513

Figure 6. Ubiquilin-1 overexpression attenuates ER stress in R6/2 animals.

Figure 6

(A) Immunoblots of equal amounts of brain lysates from 9 week-old female animals from the R6/2 and UBQ1 cross. The blots were probed for PDI, BiP, ubiquilin, and CHOP as well as for actin to monitor protein loading. (B) Quantification of the expression of the different proteins in the four mice after normalization for actin loading. (C) Immunoblot of 12-week hippocampal lysates to detect htt aggregates retained in the stacking gel. The double transgenic mice had an approximately 30% reduction in EM48 immunoreactivity after normalization for actin loading.