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. 2014 Jan 27;9(1):e87552. doi: 10.1371/journal.pone.0087552

Figure 8. Effect of inflammasome activation on macrophage migration in vitro.

Figure 8

Macrophage migration in vitro was assayed using Transwell inserts with a 5 µm porous membrane. The migratory cells on the lower side of insert membrane were quantified. BMMs in Transwell inserts were primed with LPS (1 ng/ml) for 3 h and treated with ATP (2.5 mM, 16 h), IL1β (0.5 ng/ml), MSU (100 µg/ml), or IL18 (25 nM) in the absence or presence of caspase-1 inhibitor WEHD (0.15 µg/ml) or IL1R antagonist (IL1Ra, 40 ng/ml). Quantification of the transwell assays reveals the ability of BMMs to migrate from inside Transwell inserts through the membrane upon stimulation. * P<0.05 vs. untreated Asc+/+ control group; # P<0.05 Asc−/− vs. Asc+/+ group (n = 6).