Figure 4. Circularity parameter. Line graphs show Circularity mean value ± SE at each time point in HCT-8 and HCT-8FUres cells. **P < 0.01 vs. 0 h; ^###P < 0.001 vs. 3 h; ^£££P < 0.001 vs. 6 h by ANOVA followed by Bonferroni post-test. Histogram shows Circularity mean value ± S.E., comparing HCT-8 and HCT-8FUres cell lines at each time considered. *P < 0.05; ***P < 0.001 by unpaired two-tailed t-test.