Figure 1. Isolation and primary culture of human ADSCs. The isolated process and primary culture of human ADSCs were described in the Materials and Methods. (A) The pieces of adipose tissue attached on the walls of culture flasks on the first day of culture. (B) The ADSCs demonstrated spindle-like morphology on the seventh day of culture. (C) The ADSCs with fibroblast-like morphology developed into 80–90% confluence after 10 d of primary culture. (D) One representative colony with sphere-like shape was observed in 2-week culture. Scale bars: 200 µm