Figure 2. Cell surface antigens of human ADSCs were detected by flow cytometry. The ADSCs at passage 3 were processed with FITC or PE-conjugated monoclonal antibodies to test a set of expression levels of cell surface antigens as described in the Materials and Methods. Nonspecific IgG were examined as a control (red). (A) Representative results of flow cytometry analysis, which showed that ADSCs could expressed positively CD44, CD105, CD29, CD90, and CD13. In contrast, almost no expression of the hematopoietic lineage markers of CD45, CD34, CD31 and CD106 was detected in ADSCs. (B) The geometric mean of cell surface antigens detected by flow cytometry.