Figure 3. Adipogenic and osteogenic differentiation of human ADSCs in vitro. Human ADSCs at passage 2 were cultured in the induction medium for two weeks for adipogenic differentiation, or for three weeks for osteogenic differentiation. (A) Adherent ADSCs, no staining as a negative control for adipogenic differentiation. (B) Oil red-O staining for induced ADSCs was positive, and presence of lipid droplets demonstrated that ADSCs could differentiate into adipogenic lineage. (C) Adherent ADSCs, no staining as a negative control for osteogenic differentiation. (D) Von Kossa staining showed that a calcified extracellular matrix of induced ADSCs was detected, which confirmed ADSCs could differentiate into osteogenic cells. Scale bars: 200 µm