Skip to main content
. 2014 Jan 27;9(1):e87454. doi: 10.1371/journal.pone.0087454

Figure 3. Humoral responses after subcutaneous immunization with p24 protein mixed with live or heat-killed spores.

Figure 3

Detection of anti-p24 specific IgG antibodies in serum samples of immunized BALB/c (A) and C57 BL/6 mice (B). Mouse groups (n = 5) were immunized (three doses on days 0, 14 and 28) with viable spores (▵), heat-killed spores (HkSp) (◊), p24 protein (○), p24 protein in combination with Alum (▪), p24 protein in combination with spores (•) or p24 protein in combination with heat-killed spores (▴). *** p<0.001 when compared with group immunized with p24 only or with each other. Specific IgG subclasses elicited in BALB/c (C) and C57BL/6 (D) mice after complete immunization regimen. The IgG2a/IgG1 or IgG2c/IgG1 ratios are indicated at the top of the figure. * p<0.05 or *** p<0.001. All the results are presented as the mean ± s.e.m. of three independent experiments, and the values obtained from PBS immunized mice were subtracted from each group.