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Simulated PCR phase transitions produced from SigmaPlot (version 8.0, SPSS) displaying amplification curves for a true heterozygous genotype. The theoretical behaviour of PCR amplification is determined by the initial ground (IG), exponential growth (EG), linear growth (LG) and plateau (P) phases, respectively. Based on a four-parameter sigmoid model (equation 1), the variables y0, a, x0 and b are obtained to determine ambiguous genotypes based on three criteria.
