Table 2. Results of quantitative real-time PCR analysis of the 18 confirmed CNVRs.
CNVR# | Chra | Starta | End | Type | Primer | Positive samples | Negative samples | ||||
Sample detected | Sample confirmed | Positive predictive rate | Sample detected | Confirmed samples | Negative predictive rate | ||||||
9 | 1 | 18347414 | 18379506 | loss | A2 | 3 | 3 | 1.0000 | 9 | 4 | 0.4444 |
87 | 1 | 284428678 | 284521660 | loss | CGH1-1 | 11 | 11 | 1.0000 | 1 | 1 | 1.0000 |
Gene2-2 | 11 | 11 | 1.0000 | 1 | 1 | 1.0000 | |||||
101 | 1 | 296555516 | 296570221 | loss | E1-2 | 2 | 1 | 0.5000 | 10 | 0 | 0.0000 |
234 | 11 | 62512880 | 62910688 | both | F1-2 | 10 | 10 | 1.0000 | 2 | 2 | 1.0000 |
F1-3 | 10 | 10 | 1.0000 | 2 | 2 | 1.0000 | |||||
235 | 14 | 133945595 | 134212123 | gain | Gene5-4 | 2 | 2 | 1.0000 | 10 | 1 | 0.1000 |
240 | 11 | 70582866 | 70599250 | gain | J1-3 | 1 | 1 | 1.0000 | 11 | 8 | 0.7273 |
296 | 13 | 34208583 | 34234865 | loss | CGH3-1 | 5 | 5 | 1.0000 | 7 | 7 | 1.0000 |
Gene9-4 | 5 | 5 | 1.0000 | 7 | 7 | 1.0000 | |||||
393 | 14 | 99243227 | 99268572 | loss | CGH9-1 | 3 | 2 | 0.6667 | 9 | 2 | 0.2222 |
CGH9-2 | 3 | 2 | 0.6667 | 9 | 2 | 0.2222 | |||||
CGH9-3 | 3 | 2 | 0.6667 | 9 | 2 | 0.2222 | |||||
752 | 3 | 95978937 | 95991620 | gain | CGH7-1 | 12 | 12 | 1.0000 | 0 | 0 | — |
793 | 4 | 50047123 | 50284182 | loss | E4 | 5 | 4 | 0.8000 | 7 | 3 | 0.4286 |
842 | 5 | 9900897 | 9985394 | gain | G7 | 1 | 1 | 1.0000 | 11 | 4 | 0.3636 |
870 | 5 | 60760998 | 60776722 | loss | CGH8-3 | 2 | 2 | 1.0000 | 10 | 2 | 0.2000 |
936 | 6 | 72495512 | 72520616 | gain | I2 | 2 | 2 | 1.0000 | 10 | 4 | 0.4000 |
loss | I4 | 2 | 2 | 1.0000 | 10 | 4 | 0.4000 | ||||
995 | 7 | 50302129 | 50673147 | loss | D1-1 | 1 | 1 | 1.0000 | 11 | 0 | 0.0000 |
D2-1 | 1 | 1 | 1.0000 | 11 | 0 | 0.0000 | |||||
1048 | 8 | 4481028 | 4498936 | loss | Gene3-4 | 3 | 3 | 1.0000 | 9 | 6 | 0.6667 |
1090 | 8 | 43313707 | 43894062 | gain | kit6 | 2 | 2 | 1.0000 | 10 | 0 | 0.0000 |
CGH2-1 | 2 | 2 | 1.0000 | 10 | 0 | 0.0000 | |||||
1322 | X | 91199797 | 91210413 | loss | CGH10-3 | 5 | 4 | 0.8000 | 7 | 4 | 0.5714 |
1323 | X | 91329230 | 91359396 | loss | CGH5B-1 | 6 | 6 | 1.0000 | 6 | 4 | 0.6667 |
CGH6A-2 | 6 | 6 | 1.0000 | 6 | 4 | 0.6667 | |||||
0.9321 | 0.4732 |
The Sus scrofa 10.2 assembly (http://www.ensembl.org/Sus_scrofa/Info/Index) was used to indicate the position of the CNVRs.