Table 1. Cd and Zn content and their effects on MDA and protein carbonyl contents and DNA in tobacco seedlings after exposure to Cd (10 and 15 µM), Zn (25 and 50 µM) and their combinations.
Treatment | Cd content µg g−1 DW | Zn content µg g−1 DW | MDA content µmol g−1 FW | Carbonyl content µmol mg−1 protein | % tail DNA |
control | <0.001d | 171.4±9.3c | 186.29±30.23bc | 50.12±3.87c | 2.95±0.73e |
10 µM Cd | 102.5±12.1c | 125.8±8.9c | 237.23±20.59ab | 69.73±6.20ab | 7.45±1.29bc |
15 µM Cd | 261.5±17.8a | 100.9±7.2c | 259.74±40.78a | 71.94±2.87a | 7.13±1.67cd |
25 µM Zn | <0.001d | 240.4±5.0bc | 184.36±13.63bc | 53.30±4.13c | 7.49±0.63bc |
50 µM Zn | <0.001d | 311.7±6.5ab | 203.99±24.05abc | 54.76±7.80c | 7.93±0.66abc |
10 µM Cd 25 µM Zn | 204.0±6.1ab | 356.4±3.8a | 169.66±9.78c | 55.43±6.15c | 10.63±1.36a |
10 µM Cd 50 µM Zn | 209.0±6.5ab | 354.5±3.0a | 219.01±12.67abc | 55.52±5.07c | 10.04±0.54ab |
15 µM Cd 25 µM Zn | 212.5±3.2ab | 261.1±21.0bc | 193.71±9.37bc | 59.94±3.58abc | 9.83±0.65abc |
15 µM Cd 50 µM Zn | 235.7±3.9ab | 279.8±4.6b | 193.03±12.23bc | 62.70±3.63abc | 4.54±0.56de |
Values represent means ± standard errors (n = 6). Values marked with different letters indicate significantly different treatments (P<0.05) within seedling data set (LSD multiple test).