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. 2014 Jan 27;9(1):e87724. doi: 10.1371/journal.pone.0087724

Figure 3. Size-exclusion chromatography and stoichiometry analysis.

Figure 3

(A) Size-exclusion chromatogram of affinity-purified MCA2-6H. The retention volumes of each molecular weight standard are shown with molecular weights above the panel. The inset shows the results of SDS-PAGE visualized by CBB staining of the first and second elution (E1 and E2) from the affinity column. (B) SDS-PAGE under non-reducing conditions of purified MCA2-6H followed by CBB staining. Arrowheads indicate the possible monomer, dimer, trimer, and tetramer of MCA2-6H. The bottom arrowhead shows an additional band, which may correspond to a monomer with an internal disulfide bond of MCA2-6H. (C) SDS-PAGE under reducing conditions of cross-linked MCA2-6H followed by CBB staining. Arrowheads represent the positions of the monomer, dimer, trimer, and tetramer of MCA2-6H. The time (min) of crosslinking is shown under the bottom of each lane.