Table 2. Comparison of the proposed method and the classical method based on the analysis of 50 amplification curves obtained either with CAV1 DNA or RAP1GA1 DNA, according to the same protocol as that used with IGF1 DNA (see Table 1).
| Expected number (OD measurement) | Estimated number: mean ± SD (n = 10) | Target | |
| |
Classical method |
Proposed method |
|
| 102 | (1.02 ± 0.19) × 102 | (0.75 ± 0.17) × 102 | CAV1 |
| 103 | (0.89 ± 0.10) × 103 | (0.68 ± 0.08) × 103 | CAV1 |
| 104 | (1.02 ± 0.07) × 104 | (0.75 ± 0.11) × 104 | CAV1 |
| 105 | (1.04 ± 0.09) × 105 | (0.73 ± 0.09) × 105 | CAV1 |
| 106 | (0.97 ± 0.09) × 106 | (0.64 ± 0.03) × 106 | CAV1 |
| 102 | (0.98 ± 0.27) × 102 | (0.25 ± 0.07) × 102 | RAP1GA1 |
| 103 | (1.25 ± 0.27) × 103 | (0.32 ± 0.05) × 103 | RAP1GA1 |
| 104 | (0.87 ± 0.14) × 104 | (0.25 ± 0.04) × 104 | RAP1GA1 |
| 105 | (0.94 ± 0.14) × 105 | (0.22 ± 0.02) × 105 | RAP1GA1 |
| 106 | (1.09 ± 0.13) × 106 | (0.26 ± 0.03) × 106 | RAP1GA1 |