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(A) Titers of IgG antibody against P1 and P2 in mouse sera. The titers of mice sera were determined as a series of two-fold dilutions by ELISA. (B) The immunological cross-reactivity was analyzed between mice antisera against P1 or P2 with virus-like particles (VLPs) of HBoV1, 2, 3, and 4 by Western blot. The VLPs of human parvovirus B19 and PARV4 were used as controls.
