Table 1. Target sequences and the design of molecular beacons.

Wild-type K-ras target (Bases 1–78)

5′-ATGACTGAATATAAACTTGTGGTAGTT GGAGCTGGTGGCGTAGG

caag AGTGCCTTGACGATACAGC TAATTCAGAAT-3′

K-ras dual FRET molecular beacons

Donor MB: 5′-/Cy3/CCTACGCCACCAGCTCCGTAGG/BHQ-2/-3′

Acceptor MB: 5′-/BHQ-3/AGTGCGCTGTATCGTCAAGGCACT/Cy5/-3′

Survivin target (Bases 1–78)

5′-ATGGGTGCCCCGACGTTGCCCCCTGCC TGGCAGCCCTTTCTC

aagg ACCACCGCATCTCTAC ATTCAAGAACTGGCCC-3′

Survivin dual FRET molecular beacons

Donor MB: 5′-/Cy3/GAGAAAGGGCTGCCATTCTC/BHQ-2/-3′

Acceptor MB: 5′-/BHQ-3/ACCACGTAGAGATGCGGTGGT/Cy5/-3′

‘Random’ sequence target

5′-ATCGGTGCGCTTGTCG-3′

‘Random’ sequence molecular beacons

Donor MB: 5′-/Cy3/CACGTCGACAAGCGCACCGATACGTG/BHQ-2/-3′

Acceptor MB: 5′-/BHQ-3/ACGTGCGACAAGCGCACCGATCACGT/Cy5/-3′

Molecular Beacon (MB): underlined bases, bases added to create the stem domain. Target: lowercase bold, bases between two target sequences of the donor and acceptor beacon.