Figure 6. EGCG increases gemcitabine-induced loss of mitochondrial membrane potential.

Mz-ChA-1 cells were pre-incubated with diluent controls, 1μM EGC, or 1 μM EGCG for one hour prior to incubation with either diluent alone or 30 μM gemcitabine for 24 hours. 1 × 10⁵ cells were then labeled with 10 μg/ml JC-1 for 30 minutes, and fluorescence measured. The loss of mitochondrial membrane potential was assessed as the change in ratio of red to green fluorescence with loss of mitochondrial membrane potential, causing an increase in green fluorescence. EGCG decreases the red:green fluorescence ratio during incubation with gemcitabine consistent with enhanced loss of mitochondrial membrane potential.