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. Author manuscript; available in PMC: 2014 Dec 19.
Published in final edited form as: J Phys Chem A. 2013 Dec 5;117(50):13926–13934. doi: 10.1021/jp410051w

Figure 2.

Figure 2

(A) Normalized steady-state emission spectra of three Dpo4 mutants in the apo state. The arrows indicate the fluorescence wavelengths gated in fs-resolved measurements. (B) Normalized steady-state emission spectra of the mutant Y12W at the active site in the finger domain and Y312W as a control in the little finger domain with and without binding to DNA. The inset shows a close-up view around the emission peaks and the peak differences are minor. (C) Femtosecond-resolved fluorescence transients of the three Dpo4 mutants gated at 360 nm in apo state. The symbols are the experimental data and the solid lines are the best exponential fit.