Figure 5. Cytotoxicity against RM-1. T cells isolated from C57BL/6 mice were co-cultivated with mitomycin C (MMC) treated with five types of mature DCs (Ad-tPSMA-IRES-m4-1BBL-tranduced DCs, Ad-tPSMA-transduced DCs, Ad-1BBL-tranduced DCs, Ad-eGFP-transduced DCs and none-transduced DCs, respectively) for 7 d, then treated again for another 7 d. Then T cells were harvested and used for effector cells for detecting specific cytotoxicity against target cells. The results are expressed as means ± SD of three replicates. (A) Ad-tPSMA-IRES-m4-1BBL-tranduced DCs-primed T cells showed high cytotoxicity against RM-1-PSMA cells compared with other four types of DCs-primed T cells (*p < 0.05). (B) Five types of DCs-primed T cells do not demonstrate detectable cytotoxic activities against the RM-1 cells used as a target control. All experiments were done in triplicate.