Table 2. Putative Cathepsin L sites within HLA-DR binding peptides.
| Entinostat | ||
|---|---|---|
| Peptide | Cleavage site score | Site specificity |
| PSKGPLQSVQVF | 190 | > 95% |
| AEREIVRDIKEKL | 98 | > 95% |
| FERNKAIKVIIA | 11 | < 95% |
| QPVNPRSLEKLEIIPASQF | 1792 | > 99% |
| LNKSINPDEAVAYGAA(V) | 17 | > 95% |
| LPVILDIIKGEMSRP | 21 | > 95% |
| ASAFAFLAPARAIL | 9 | > 95% |
| Average score | 67.2 | 16/13 |
| DMSO | ||
| DKNNYQQLLGALDYS | 521 | > 99% |
| EHAGVISVL | 10 | < 95% |
| LANIAILNNNLNTL | 0.2 | < 95% |
| SDVGEFRAVTELGRPA | 579 | > 99% |
| GYKAFSSLLASSAVSPE | 343 | > 99% |
| PLNDNIATLLHQSSD | 383 | > 99% |
| LPLKFSNTLELK | 62 | > 95% |
| APPPYSSISAESAAY | 223 | > 99% |
| GMAFTFLKVLNNME | 109 | > 95% |
| AQENIKTCEYVSE(G) | 4 | < 95% |
| NTNDFVTLRTPEGLPDAP | 23 | < 95% |
| VAYVQQIFVKSSVF | 147 | > 95% |
| IKEFGSLPTTPSEQ | 455 | > 99% |
| Average score | 207.2 | |
| p-value for average scores | < 0.03 | 36/27 |
Note: Cathepsin L1 cleavage sites (in bold) and scores obtained using SitePrediction (www.dmbr.ugent.be/prx/bioit2-public/SitePrediction/).