Figure 5.

PAT Characteristics in erg28 Plants.

(A) Defective PAT in erg28R1-2 and erg28T1-2 plants. Thirty-five-day-old erg28R1-2, erg28T1-2 developing usable inflorescence stems, and wild-type (WT) plants were used to determine IAA transport using tritium-labeled IAA in the presence or absence of NPA (15 μM) (error bars are sd; n = 5).

(B) Expression of four NPA-responsive genes (indicated by their AGI codes) in erg28R1-1, erg28R1-2, erg28R1-4, erg28R1-6, erg28T1-1, erg28T1-2, erg28T1-4, and erg28T1-6 plants. Thirty-five-day-old plants were used for qRT-PCR analysis. Data represent the fold change over the wild type (error bars are sd; n = 3).

(C) IAA content in 35-d-old wild-type, erg28R1-2, and erg28T1-2 plants (error bars are sd; n = 3). FW, fresh weight.

(D) Alteration of the DR5:GUS auxin reporter gene in erg28 background. Leaves from 35-d-old DR5:GUS (left, control) and erg28T1-2/DR5:GUS (middle and right) plants were used. Bars = 0.25 cm.

(E) Immunolocalization of PIN1 using anti-At-PIN1 in roots of 10-d-old erg28R1-2 (left) and erg28T1-2 (middle) plants. PIN1 localizes to the basal side of the cell, as shown in PIN1-GFP plant (right) used as a control. Bars = 5 μm.