Figure 6.

Impaired PAT Phenotypes of erg28 Plants Correlate with CMMC Accumulation.

(A) UPLC-MS/MS single ion recording (SIR) chromatogram at m/z 453 shows the presence of CMMC (arrowhead) in lipid extracts of 35-d-old erg28R1-1, erg28T1-2, and erg28R1-6 but not in 35-d-old wild-type (WT) plants and shows the authentic CMMC standard (arrow, inset: chemical structure). Detailed analysis using MRM mode, product ion spectra, and the fragmentation pathways are shown in Supplemental Figures 10 to 13 online. RI, relative intensity.

(B) Quantification of CMMC in erg28R1 and erg28T1 plants (error bars are sd; n = 3).

[See online article for color version of this figure.]