Table 5.

Pairwise genetic distances (uncorrected p) of 16S DNA sequences among members of the Hypsiboas calcaratus species complex. Mean ± SD is given with range in parentheses (below diagonal). Number of individuals compared is shown above diagonal. Diagonal shows intra-clade genetic distances.

	Clade A Hypsiboas fasciatus	Clade B Hypsiboas almendarizae	Clade C Hypsiboas calcaratus	Clade D Hypsiboas maculateralis	Clade E Hypsiboas alfaroi	Clade F Hypsiboas tetete	Clade G	Clade H	Clade I	Clade J	Clade K
Clade A Hypsiboas fasciatus	0.004 ± 0.004 (0.007–0.017)	n = 31	n = 49	n = 36	n = 45	n = 18	n = 17	n = 19	n = 17	n = 19	n = 16
Clade B Hypsiboas almendarizae	0.036 ± 0.009 (0.035–0.049)	0.008 ± 0.0006 (0.001–0.012)	n = 50	n = 37	n = 46	n = 19	n = 18	n = 20	n = 18	n = 20	n = 17
Clade C Hypsiboas calcaratus	0.057 ± 0.011 (0.038–0.078)	0.041 ± 0.003 (0.034–0.050)	0.009 ± 0.008 (0.001–0.031)	n = 55	n = 64	n = 37	n = 36	n = 38	n = 36	n = 38	n = 35
Clade D Hypsiboas maculateralis	0.077 ± 0.003 (0.072–0.100)	0.074 ± 0.003 (0.069–0.086)	0.086± 0.006 (0.064–0.102)	0.002 ± 0.0008 (0.001–0.004)	n = 51	n = 24	n = 23	n = 25	n = 23	n = 25	n = 22
Clade E Hypsiboas alfaroi	0.082 ± 0.004 (0.074–0.100)	0.080 ± 0.003 (0.072–0.090)	0.089 ± 0.004 (0.073–0.105)	0.107 ± 0.004 (0.097–0.120)	0.002 ± 0.002 (0.003–0.017)	n = 33	n = 32	n = 34	n = 32	n = 34	n = 31
Clade F Hypsiboas tetete	0.085 ± 0.002 (0.083–0.095)	0.078 ± 0.001 (0.073–0.082)	0.084 ± 0.003 (0.074–0.096)	0.100 ± 0.002 (0.093–0.107)	0.028 ± 0.002 (0.023–0.037)	0.013 ± 0.009 (0.001–0.019)	n = 5	n = 7	n = 5	n = 7	n = 4
Clade G	0.082 ± 0.002 (0.081–0.092)	0.077 ± 0.002 (0.073–0.079)	0.085 ± 0.003 (0.075–0.091)	0.097 ± 0.003 (0.091–0.107)	0.039 ± 0.002 (0.033–0.045)	0.041 ± 0.001 (0.039–0.043)	0	n = 6	n = 4	n = 6	n = 3
Clade H	0.088 ± 0.003 (0.083–0.101)	0.082 ± 0.002 (0.077–0.087)	0.095 ± 0.003 (0.088–0.108)	0.098 ± 0.003 (0.094–0.107)	0.055 ± 0.003 (0.050–0.068)	0.053 ± 0.002 (0.049–0.056)	0.052 ± 0.002 (0.050–0.055)	0.004 ± 0.002 (0.001–0.007)	n = 6	n = 8	n = 5
Clade I	0.088 ± 0.004 (0.081–0.097)	0.083 ± 0.003 (0.077–0.089)	0.098 ± 0.004 (0.093–0.115)	0.097 ± 0.005 (0.091–0.112)	0.059 ± 0.002 (0.056–0.067)	0.056 ± 0.001 (0.055–0.058)	0.058 ± 0.001 (0.057–0.059)	0.031 ± 0.002 (0.028–0.034)	0.003	n = 6	n = 3
Clade J	0.081 ± 0.002 (0.077–0.087)	0.082 ± 0.003 (0.079–0.090)	0.084 ± 0.004 (0.079–0.097)	0.094 ± 0.004 (0.091–0.108)	0.053 ± 0.004 (0.049–0.075)	0.052 ± 0.002 (0.048–0.055)	0.035 ± 0.0008 (0.034–0.035)	0.038 ± 0.005 (0.031–0.045)	0.056 ± 0.002 (0.054–0.058)	0	n = 5
Clade K	0.073 ± 0.002 (0.071–0.081)	0.068 ± 0.001 (0.065–0.071)	0.072 ± 0.008 (0.047–0.085)	0.051 ± 0.004 (0.048–0.059)	0.101 ± 0.004 (0.094–0.112)	0.099 ± 0.001 (0.098–0.100)	0.097	0.102 ± 0.002 (0.100–0.105)	0.103	0.086 ± 0.0002 (0.085–0.086)	0