Figure 3.

Effects of BPA treatment on protein levels of the adipogenic markers aP2 and perilipin. Human preadipocytes were differentiated as described with increasing concentrations of BPA for 14 days, and protein levels of the adipogenic marker aP2 were assessed by western blotting (a) and densitometry (b) analyses. Human preadipocytes were also differentiated in the presence of 25 μM BPA or vehicle control for 6 or 14 days and protein levels of the adipogenic markers aP2 (c, d) and perilipin (e, f) were assessed by western blotting and densitometry analyses. (g) Cells were differentiated in the presence of 1 μM DEX, and protein levels of the adipogenic markers aP2 and perilipin were assessed at days 0, 6 and 14 of differentiation by western blotting analysis. Images are representative of at least three separate experiments. β-Actin was used as the gel loading control for all blots. All densitometry values are expressed as means±s.e.m. for at least three experiments. *P<0.05, **P<0.01 relative to control calculated by one-way analysis of variance with Holm–Sidak post-test analysis.