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. 2014 Jan 28;8:10. doi: 10.3389/fncel.2014.00010

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Copyright © 2014 Young, Lafourcade, Platel Lin and Bordey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Striatal neurons project dendrites into the SVZ. (A) Images of single optical sections at different depths (indicated in the right corner) from the surface of the sagittal slice. Two striatal neurons were filled with Alexa Fluor-568 (pseudo-colored green) and the fluorescent dye Fluo-4AM (pseudo-colored red) was pressure applied in the SVZ. Not every SVZ cell is Fluo-4-positive. Images are overlaid on the IR-DIC. (B) 3D Z-projection using the blend mode in Imaris illustrating the 3D shape of the SVZ in a sagittal section. (C) 3D Y-projection of the image in B following a rotation up around the X axis. (D) Maximum intensity projection in Imaris. (E) Rreconstruction of one of the filled neuron with orange overlaid to marked the processes entering the SVZ. Scale: 30 μm (A,D,E).