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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Endod. 2013 Aug 31;40(2):255–260. doi: 10.1016/j.joen.2013.07.009

Figure 2.

Figure 2

(A) A representative CLSM image of MPDC-23 cells that were triple-stained with ethidium homodimer III (Etd; red-fluorescent non-vital DNA dye), Heoschst 33342 (blue-fluorescent nuclear counter-stain), and fluorescein isothiocyanate-annexin V (FITC-AnV; green-fluorescent phosphatidylserine-binding cytoplasmic dye) after their exposure to the test cements. Healthy cell nuclei are stained blue, apoptotic cells show green cytoplasm, necrotic cells show red or pink nuclei. (B) A representative 2-dimensional flow cytometry dot plot of the data derived from FITC-AnV and Etd-stained MDPC-23 cells after their exposure to test cements for 3 days. (C) A bar graph comparing the percentage of vital cells (lower left quadrant of Figure 2B) after exposure to the test cements (N = 3). Groups labeled with the same letter designators are not significantly different (P > 0.05).