Figure 3.

Tumour necrosis factor-related apoptosis-inducing ligand receptor (TRAIL-R) expression is reduced in wild-type (WT) mice fed dextran sulphate sodium (DSS). Eight-week-old WT mice were administered 2·5% DSS or vehicle in their drinking water for 5 days. (a) Immunohistochemical staining of colon tissues (brown, TRAIL-R; blue, haematoxylin stain). (b) Real-time PCR analysis for the expression of TRAIL-R in colon epithelial cells (CEC) of the mice fed with DSS for 0 and 5 days. (c) Western blot analysis of the expression of TRAIL-R in CEC. Cell lysates were pooled from two mice that were fed with DSS for 0 days, 2 days and 5 days.