Figure 2.

Negative selection of ^QM11T-cell receptor transgenic (^QM11TCR-Tg) T cells occurred normally, but CD8^low+ idiotype^high+ cells failed to develop in aly/aly mice. (a) The thymocyte numbers of ^QM11TCR-Tg, aly/aly or aly/+ mice with indicated H-2 backgrounds are shown. Five to six mice aged 8 to 10 weeks for each strain were analysed. Error bars represent SD. (b) The expression of CD4 and CD8 on the thymocytes from ^QM11TCR-Tg, aly/aly (right) or aly/+ (left) mice with negatively selecting H-2^bxk background is indicated. A representative set of results from the analyses of > 10 mice is shown. (c) The expression of CD8 and idiotype on the splenocytes from ^QM11TCR-Tg, aly/aly (right) or aly/+ (left) mice on an H-2^s background are shown. A representative set of results from analyses of > 10 mice is shown. The percentage of CD8^low+ idiotype^high+ cells in aly/aly mice varied from 1/20 to 1/3 of that in aly/+ mice.