Figure 1.

IGKV-IGKJ5 multiplex PCR with DNA from the human pre-B cell line 697 after induction with lipopolysaccharide (LPS) or interleukin-1 (IL-1). Genomic DNA was isolated and IGKV-IGKJ rearrangements were amplified by PCR with the IGKV1f/6, IGKV2f and IGKV3f forward primers and the Jk5 reverse primer.³² A schematic diagram of the organization of the native haplotype carrying the IGKV1-8-IGKJ4 rearrangement in the 697 cell line is shown on the left (EMBL accession number HF674726). New IGKV-IGKJ5 rearrangements result in a smear migrating between 120 and 300 bp (right). After longer periods of induction, the signal for the native IGKV1-8-IGKJ4 disappeared and the signal from the new IGKV3-IGKJ5 became more intense (see Supplementary material, Fig. S1).