Figure 5.
Interleukin (IL)-33 is induced in dendritic cells (DCs) via MyD88 (myeloid differentiation primary-response protein 88) signaling pathways. (a) The mRNA expression of nuclear factor (NF)-κB1, NF-κB2, RelA, and IL-33 was evaluated by quantitative real-time PCR in DCs from MyD88+/+ and MyD88−/− mice exposed to flagellin (10 µg ml−1) for 8 h. (b) Representative images showing IL-33 production by immunofluorescent staining (Green) with propidium iodide counterstaining (Red) in DCs from MyD88+/+ and MyD88−/− mice treated with flagellin (10 µg ml−1) for 24 h. Each bar in the diagrams represents mean ± s.d. of three independent experiments. *P<0.05; **P<0.01, compared with MyD88+/+ control, ^P<0.05; ^^P<0.01, compared with MyD88+/+ flagellin.