Figure 1. MLN cell proliferation after stimulation with total H. polygyrus ES (ESAg) and S antigen (SAg) and fraction 9 (F9). The antigen effect on stimulation of MLN cell proliferation was calculated using the formula: Proliferation % = (ODAg/ODM) × 100. Where (ODAg) indicates the optical density of the tested antigen and (ODM) indicates the optical density of the control sample with medium alone. Cell proliferation was assayed daily. The experiments were done in triplicate. Bars represent the mean ± SE of six mice of a representative experiment (n = 6). Statistical significance between groups (control and infected) was assessed by ANOVA. ap < 0,05 compared with untreated cells (MEDIUM) within the same group; bp < 0,05 compared with cells with other group treated by the same manner.