Figure 4. Effects of H. polygyrus infection on CD4 T cell-cycle progression. CD4⁺ T cells were negatively selected by magnetic field-assisted cell sorting. FACS profiles of cells from control mice (A) and infected mice (B) stained with PI for DNA content are shown. A cell cycle-DNA content analysis was performed. The number on each panel shows the percentage of hypodiploid nuclei. 20000 CD4⁺ T cells were analyzed for PI fluorescence on a Becton Dickinson FACScalibur.