Figure 8. Expression of apoptosis- related proteins; survivin, cyclin dependent kinase inhibitor p27Kip1 by pure CD4 lymphocytes of control mice and infected with H. polygyrus mice. Ct data for p27 Kip1 protein and survivin have been normalized to the housekeeping genes GAPDH and corrected for the total control mean value using Light Cycler Nano Software-1.0. The mean fold change and standard error of target gene expression were calculated from three independent biological replicates. Statistical significance between groups (control and infected) was assessed by ANOVA. ^ap < 0,05 compared with untreated cells (MEDIUM) within the same group; ^bp < 0,05 compared with cells with other group treated by the same manner.