Table 1.
dA-AL-I adduct levels in human freshly frozen and FFPE kidney tissue
| Subject | Tissue |
32P-postlabeling dA-AL-I / 108 bases |
LC-MS/MS3 dA-AL-I /108 bases |
Surface area of tissue in block (cm2) |
DNA yield, µg per 2 FFPE 10 µm-section cuts |
|---|---|---|---|---|---|
| 1 | FR | 6.5 | 7.0 ± 0.2a | - | |
| FFPE | n.a. | 6.1 | 1.8 | 3.0 | |
| FFPE | n.a. | 4.6 | 1.8 | 4.8 | |
| FFPE | n.a. | 4.7 | 1.8 | 4.0 | |
| FFPE | n.a. | 6.2 | 1.8 | 7.2 | |
| 2 | FR | 3.2 | 2.7 ± 0.1b | - | |
| FFPE | n.a. | 1.8 | 1.6 | 2.5 | |
| 3 | FR | 2.2 | 3.9 ± 0.1b | - | |
| FFPE | n.a. | 3.8 | 1.5 | 7.5 | |
| FFPE | n.a. | 3.7 | 1.5 | 8.0 | |
| 4 | FR | n.d. | 1.0 ± 0.1b | - | |
| FFPE | n.a. | 1.5 | 0.9 | 5.3 | |
| FFPE | n.a. | 1.6 | 0.9 | 4.9 | |
| 5 | FR | n.d. | 0.13 ± 0.02b,c | - | |
| FFPE | n.a. | 0.48 | 1.0 | 3.3 | |
a
Independent triplicate measurements;
b
independent duplicate measurements; other samples were measured in singlicate;
c
above the limit of detection (~1 adduct per 109 bases by UPLC-ESI-MS/MS3);
n.a. = not analyzed; n.d. = not detected, below the limit of detection of ~1 adduct per 108 bases by 32P-postlabeling; FR= freshly frozen tissue; FFPE = formalin fixed paraffin embedded tissue.