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. Author manuscript; available in PMC: 2014 Jan 28.
Published in final edited form as: Stem Cells. 2013 Apr;31(4):666–681. doi: 10.1002/stem.1302

Figure 3.

Figure 3

miR-302 cluster directly regulates MBD2. (A): Expression levels of potential target genes in hES-H9, CB-iPSCs, partially reprogrammed cells, and cord blood CD34+(CB-CD34+) were analyzed by real-time PCR. Relative expression values of mRNAs are normalized to the level of ACTIN mRNA. (B): Vector alone (psi-check) or luciferase-reporter constructs containing WT 3′UTR of MBD2, MeCP2, and Tet (WT) or mutant 3′UTR (Mut) were transfected into hECCs which expressed miR-302 cluster. (C): Effect of miR-302 cluster on relative luciferase activities is presented as histograms. Experiments were carried out in triplicates for each sample and values represent mean ± SD. Student’s t test: ***, p < .001. Abbreviations: CB-iPSC, cord blood-derived iPS; hES, human embryonic stem; iPSCs, induced pluripotent stem cells.