Table 1.

Primer pairs used for PCR amplification of Pnpla2/PNPLA2 transcripts.

Primer	Primer Sequencesa	Templateb	Expected PCR
Pair Name			Product Size (bp)c
PPB	5’-tgtggcctcattcctcctac-3’	PNPLA2	217
	5’-tcgagaggcggtagagattg-3’	PNPLA2Δ4a	115
PPC	5’-tccgagagatgtgcaaacag-3’	PNPLA2	361
	5’-aaacggatggtgaaggacac-3’	PNPLA2Δ6	193
PPD	5’-tgtggcctcattcctcctac-3’	PNPLA2	608
		PNPLA2Δ4a	506
	5’-aaacggatggtgaaggacac-3’	PNPLA2Δ6	440
		PNPLA2Δ4aΔ6	338
PPH	5’-aacgccactcacatctacg-3’	PNPLA2	1025
		PNPLA2Δ4a	923
		PNPLA2Δ6	857
	5’-accagatactggcagatgct-3’	PNPLA2Δ4aΔ6	755
		PEDF-R	1019
		PEDF-RΔE5b	929
PPI	5’-atccctccttcaacctggt-3’	PNPLA2	886
		PNPLA2 Δ4a	784
		PNPLA2 Δ6	718
	5’-accagatactggcagatgct-3’	PNPLA2 Δ4aΔ6	616
		PEDF-R	880
		PEDF-RΔE5b	790
PPJ	5’-atccctccttcaacctggt-3’	PNPLA2	808
		PNPLA2 Δ4a	706
	5’-aaacggatggtgaaggacac-3’	PNPLA2 Δ6	640
		PNPLA2 Δ4aΔ6	538

^aSequence of forward (top) and reverse (bottom) primers for each primer pair. (Note, PPB and D have the same forward primer, PPC and D have the same reverse primer, and PPH and PPI have the same reverse primer) Bolded and underlined letters indicate base pairs, which deviate in the human PNPLA2 sequence in PPH and I.

^bPotential Pnpla2/PNPLA2 transcripts expected to be amplified by each primer pair. Pnpla2, full-length mouse Pnpla2 transcript. Pnpla2Δ4a, mouse cDNA of a Pnpla2 splice variant without exon 4a (E4a). Pnpla2A6, mouse cDNA of a Pnpla2 splice variant without exon 6 (E6). Pnpla2Δ4aΔ6, mouse cDNA of a Pnpla2 splice variant without E4a or E6. PEDF-R, expression vector containing full-length human PNPLA2 cDNA. PEDFRΔE5b, expression plasmid containing human PNPLA2 cDNA lacking exon 5b.

^cExpected PCR product sizes, in base pairs, resulting from amplification with the indicated primer pair and the indicated Pnpla2/PNPLA2 transcript.